5YH4
Miraculin-like protein from Vitis vinifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.040, 59.600, 74.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.930 - 1.300 |
R-factor | 0.17492 |
Rwork | 0.174 |
R-free | 0.18824 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zc8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.355 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.930 |
High resolution limit [Å] | 1.300 |
Number of reflections | 50318 |
<I/σ(I)> | 11.9 |
Completeness [%] | 100.0 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M MES buffer (pH 6.5), 12% PEG 20000 |