5YH0
The structure of DrFam20C1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 114.925, 135.988, 219.971 |
Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
Resolution | 47.843 - 3.450 |
R-factor | 0.2259 |
Rwork | 0.225 |
R-free | 0.27850 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.003 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.450 |
Number of reflections | 88916 |
<I/σ(I)> | 8 |
Completeness [%] | 99.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 18% w/v Polyethylene glycol monomethyl ether 2,000 |