5YGM

Monomeric structure of concanavalin A at pH 7.5 from Carnivalia ensiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	62.303, 86.590, 89.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.760 - 1.600
R-factor	0.1673
Rwork	0.166
R-free	0.19648
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c57
RMSD bond length	0.026
RMSD bond angle	2.326
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.760	1.680
High resolution limit [Å]	1.600	1.600
Number of reflections	32123	4323
<I/σ(I)>	28.2
Completeness [%]	98.8	92.8
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEK6K, MPD in HEPES, pH 7.5