5YGH

Crystal Structure of the Capsid Protein from Zika Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.7293
Spacegroup name	P 63
Unit cell lengths	69.417, 69.417, 58.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.792 - 1.884
R-factor	0.2073
Rwork	0.206
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sfk
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.880	1.900
Rmerge	0.098	0.743
Rpim	0.031	0.193
Number of reflections	13611
<I/σ(I)>	21.137	6.401
Completeness [%]	99.2	100
Redundancy	16	16.7
CC(1/2)	0.994	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.01M Iron (III) chloride hexahydrate, 0.1M Sodium citrate pH5.6, 10% v/v Jeffamine M-600