5YGE

ArgA complexed with AceCoA and glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.97624
Spacegroup name	P 41 21 2
Unit cell lengths	73.728, 73.728, 187.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.170 - 2.039
R-factor	0.1981
Rwork	0.197
R-free	0.22090
RMSD bond length	0.010
RMSD bond angle	1.241
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.039	5.540	2.040
Rmerge	0.102	0.054	0.463
Rmeas	0.109	0.058	0.496
Rpim	0.037	0.021	0.173
Number of reflections	33893	1900	1637
<I/σ(I)>	8.2
Completeness [%]	99.9	99.2	99.5
Redundancy	8.3	7.7	7.8
CC(1/2)		0.997	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	289	0.08 M sodium cacodylate, pH6.2, 0.16 M magnesium acetate tetrahydrate, 12% (w/v) PEG10000, 20% (w/v) glycerol