5YGC

Crystal structure of Drosophila melanogaster Papi extended Tudor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 65 2 2
Unit cell lengths	77.808, 77.808, 144.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.578 - 2.000
R-factor	0.1831
Rwork	0.180
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fdr
RMSD bond length	0.009
RMSD bond angle	1.341
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.330
Number of reflections	18114
<I/σ(I)>	36.2	4
Completeness [%]	99.9	99.9
Redundancy	23.5	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.8 M Succinic acid pH 7.0