5YGB
Crystal structure of drosophila melanogaster Papi extended Tudor domain mutant - D287A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 41 |
| Unit cell lengths | 72.237, 72.237, 50.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.393 - 1.400 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.240 |
| Number of reflections | 51043 | |
| <I/σ(I)> | 48.1 | 7.2 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 11.8 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.2 M Sodium malonate pH 7.0, 20% (W/V) PEG 3350 |
