5YG8

Crystal structure of ribose-1,5-bisphosphate isomerase from Pyrococcus horikoshii OT3 in complex with ribulose-1,5-bisphosphate, AMP and GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-02-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31 1 2
Unit cell lengths	98.590, 98.590, 256.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.380 - 2.800
R-factor	0.1586
Rwork	0.155
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yfj
RMSD bond length	0.013
RMSD bond angle	1.750
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.380	2.940
High resolution limit [Å]	2.800	2.800
Rmerge	0.145	0.507	0.055
Rmeas	0.152	0.534	0.059
Rpim	0.048	0.169	0.019
Number of reflections	35596	4669	1070
<I/σ(I)>	13.6	4.9
Completeness [%]	100.0	100	99.9
Redundancy	9.9	9.9	9.2
CC(1/2)	0.996	0.956	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.7 M NaCl, 3% PEG 6000, 25% MPD