5YF8

Crystals structure of Classical swine fever virus NS5B (residues 1-672, Y471A-E472A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	115.784, 115.784, 393.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.906 - 3.396
R-factor	0.2321
Rwork	0.230
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjq
RMSD bond length	0.010
RMSD bond angle	1.164
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	3.520
High resolution limit [Å]	3.396	7.320	3.400
Rmerge	0.128	0.030	0.523
Rmeas	0.142	0.033	0.582
Rpim	0.059	0.014	0.251
Number of reflections	18873	1972	1850
<I/σ(I)>	6.3
Completeness [%]	98.8	94.8	99.5
Redundancy	5.3	5.1	5.1
CC(1/2)		0.999	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	289	SOKALAN CP42