5YF7

Crystals structure of Classical swine fever virus NS5B (residues 1-672)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	162.249, 162.249, 56.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.351 - 2.270
R-factor	0.2011
Rwork	0.199
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjq
RMSD bond length	0.007
RMSD bond angle	0.826
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.270	4.890	2.270
Rmerge	0.075	0.043	0.490
Rmeas	0.078	0.045	0.510
Rpim	0.021	0.013	0.138
Total number of observations	450669
Number of reflections	35366	3744	3416
<I/σ(I)>	6.4
Completeness [%]	99.2	97.7	98.3
Redundancy	12.7	12.1	12.4
CC(1/2)		0.999	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	289	Polypropylene glycol 400