5YF5

Crystals structure of Classical swine fever virus NS5B (residues 1-694)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	160.029, 160.029, 55.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.397 - 2.490
R-factor	0.1882
Rwork	0.185
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjq
RMSD bond length	0.008
RMSD bond angle	0.918
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.590
High resolution limit [Å]	2.490	5.380	2.500
Rmerge	0.080	0.042	0.519
Rmeas	0.087	0.046	0.562
Rpim	0.033	0.018	0.212
Number of reflections	25700	2738	2510
<I/σ(I)>	9.9
Completeness [%]	99.7	97.9	100
Redundancy	6.7	6.2	6.8
CC(1/2)		0.998	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	289	Polypropylene glycol 400