5YF5
Crystals structure of Classical swine fever virus NS5B (residues 1-694)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 160.029, 160.029, 55.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.397 - 2.490 |
R-factor | 0.1882 |
Rwork | 0.185 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cjq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.918 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 2.590 |
High resolution limit [Å] | 2.490 | 5.380 | 2.500 |
Rmerge | 0.080 | 0.042 | 0.519 |
Rmeas | 0.087 | 0.046 | 0.562 |
Rpim | 0.033 | 0.018 | 0.212 |
Number of reflections | 25700 | 2738 | 2510 |
<I/σ(I)> | 9.9 | ||
Completeness [%] | 99.7 | 97.9 | 100 |
Redundancy | 6.7 | 6.2 | 6.8 |
CC(1/2) | 0.998 | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 289 | Polypropylene glycol 400 |