5YF4
A kinase complex MST4-MOB4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.469, 33.997, 61.023 |
| Unit cell angles | 90.00, 122.67, 90.00 |
Refinement procedure
| Resolution | 30.038 - 1.897 |
| R-factor | 0.1572 |
| Rwork | 0.153 |
| R-free | 0.19770 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.965 |
| High resolution limit [Å] | 1.897 | 1.897 |
| Rmerge | 0.109 | 0.798 |
| Rmeas | 0.119 | 0.881 |
| Rpim | 0.046 | 0.365 |
| Number of reflections | 12086 | 1123 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 98.9 | 99 |
| Redundancy | 6.4 | 5.5 |
| CC(1/2) | 0.715 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES, pH 7.5, 30% PEG 1000 |
