5YEZ
Regulatory domain of HypT M206Q mutant from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.204, 67.326, 192.255 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.479 - 2.600 |
R-factor | 0.2366 |
Rwork | 0.234 |
R-free | 0.29070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ydo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.551 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.083 | |
Rpim | 0.029 | 0.239 |
Number of reflections | 20398 | 933 |
<I/σ(I)> | 18.72 | |
Completeness [%] | 95.9 | 90.3 |
Redundancy | 7.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 | 0.1 M sodium citrate (pH 5.6) 2 % (v/v) tacsimate (pH 5.0), and 18% (v/v) PEG 3350. |