5YEU

Structural and mechanistic analyses reveal a unique Cas4-like protein in the mimivirus virophage resistance element system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	79.259, 93.453, 123.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.252 - 3.001
R-factor	0.2255
Rwork	0.224
R-free	0.26340
RMSD bond length	0.002
RMSD bond angle	0.595
Data reduction software	PHENIX
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.252	3.159
High resolution limit [Å]	3.001	3.001
Number of reflections	18840
<I/σ(I)>	10.339
Completeness [%]	99.7
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM magnesium chloride, 20% PEG 3350