5YET
Structure of R354_WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.846, 57.809, 99.948 |
| Unit cell angles | 90.00, 108.39, 90.00 |
Refinement procedure
| Resolution | 48.949 - 2.806 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.24160 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.495 |
| Data reduction software | PHENIX |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.949 |
| High resolution limit [Å] | 2.806 |
| Number of reflections | 22531 |
| <I/σ(I)> | 13.05 |
| Completeness [%] | 85.5 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200mM ammonium tartrate, 20% PEG 3350 |
