5YEQ
The structure of Sac-KARI protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-26 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.434, 90.827, 154.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.062 - 1.750 |
R-factor | 0.17662 |
Rwork | 0.175 |
R-free | 0.21585 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xdz |
RMSD bond length | 0.021 |
RMSD bond angle | 2.050 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.250 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 69217 | 6734 |
<I/σ(I)> | 33.8 | |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 4.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 0.2 M Li2SO4 and 20% PEG3350 |