5YDU
Crystal structure of Utp30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.364, 90.880, 157.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.939 - 2.646 |
| R-factor | 0.2036 |
| Rwork | 0.200 |
| R-free | 0.26550 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.646 | 2.646 |
| Rmerge | 0.168 | 0.765 |
| Number of reflections | 15291 | 1432 |
| <I/σ(I)> | 21.4 | 2 |
| Completeness [%] | 98.7 | 95.4 |
| Redundancy | 11.4 | 8.4 |
| CC(1/2) | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.45 M tri-sodium citrate dehydrate and 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5 |
