5YDO
Regulatory domain of HypT from Salmonella typhimurium (apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97960 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.344, 63.344, 100.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.441 - 2.000 |
R-factor | 0.2069 |
Rwork | 0.204 |
R-free | 0.25360 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.553 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.207 |
Rpim | 0.013 | 0.061 |
Number of reflections | 14482 | 677 |
<I/σ(I)> | 41.23 | 6.29 |
Completeness [%] | 99.3 | 95.6 |
Redundancy | 16.5 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 50mM ammonium sulfate, 50mM Bis-Tris (pH 6.5), 30%(v/v) pentaerythritol ethoxylate (15/4 EO/OH), and 2mM TCEP. |