5YDO
Regulatory domain of HypT from Salmonella typhimurium (apo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.344, 63.344, 100.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.441 - 2.000 |
| R-factor | 0.2069 |
| Rwork | 0.204 |
| R-free | 0.25360 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.553 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.207 |
| Rpim | 0.013 | 0.061 |
| Number of reflections | 14482 | 677 |
| <I/σ(I)> | 41.23 | 6.29 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 16.5 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 50mM ammonium sulfate, 50mM Bis-Tris (pH 6.5), 30%(v/v) pentaerythritol ethoxylate (15/4 EO/OH), and 2mM TCEP. |
