5YDG
Crystal structure of the Arabidopsis thaliana chloroplast RNA editing factors 2(MORF2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.209, 85.880, 86.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.644 - 2.405 |
R-factor | 0.2059 |
Rwork | 0.204 |
R-free | 0.24730 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.903 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (MR) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.106 | 0.053 | 0.445 |
Rmeas | 0.124 | 0.063 | 0.522 |
Rpim | 0.063 | 0.033 | 0.266 |
Number of reflections | 11069 | 1207 | 1118 |
<I/σ(I)> | 7.4 | ||
Completeness [%] | 95.4 | 94.1 | 99.7 |
Redundancy | 3.7 | 3.4 | 3.8 |
CC(1/2) | 0.993 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M Bis-Tris pH 6.5, 25% (w/v) PEG 3350 |