5YDF
Crystal structure of a disease-related gene, hCDC73(1-100)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.590, 53.504, 65.734 |
Unit cell angles | 90.00, 95.66, 90.00 |
Refinement procedure
Resolution | 19.489 - 1.399 |
R-factor | 0.1372 |
Rwork | 0.135 |
R-free | 0.17420 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.361 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.500 |
High resolution limit [Å] | 1.400 |
Number of reflections | 38451 |
<I/σ(I)> | 41.92 |
Completeness [%] | 98.9 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Tris, magnesium chloride | |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Tris, magnesium chloride |