5YDD
Crystal structure of C-terminal domain of Rv1828 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.035, 31.452, 76.998 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 27.240 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ydc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.020 | 0.460 |
| Number of reflections | 33255 | |
| <I/σ(I)> | 23.3 | 1.6 |
| Completeness [%] | 96.2 | 93.6 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.2 M Ammonium sulfate, 0.1 M Bis-Tris (pH 6.5) |
