5YD0
Crystal structure of Schlafen 13 (SLFN13) N'-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.968, 134.065, 77.587 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 35.236 - 3.182 |
| R-factor | 0.2027 |
| Rwork | 0.200 |
| R-free | 0.24690 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Number of reflections | 28393 | 4511 |
| <I/σ(I)> | 17.08 | 2.05 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3.41 | 3.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM MES, 20% PEG6000, 850-950 mM lithium chloride |
