5YCT
Engineered hairpin loop3 mutant monomer in Single-chain Monellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.601, 48.103, 62.712 |
| Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
| Resolution | 24.052 - 1.851 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iv7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.052 | |
| High resolution limit [Å] | 1.851 | 1.851 |
| Rmerge | 0.017 | 0.195 |
| Number of reflections | 17514 | |
| <I/σ(I)> | 24 | 3.1 |
| Completeness [%] | 98.8 | 89.04 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.999 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 8-12% (wt/vol) PEG 8000, 50mM sodium phosphate, pH 6.4-7.2, Crystals grew to maximum size in 4-5 days |
