5YCO

Complex structure of PCNA with UHRF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	H 3
Unit cell lengths	202.514, 202.514, 123.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.312 - 2.199
R-factor	0.2048
Rwork	0.204
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vkx
RMSD bond length	0.010
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.051	0.028	0.467
Rmeas	0.067	0.037	0.621
Rpim	0.043	0.023	0.405
Total number of observations	199263
Number of reflections	94479	8999	9558
<I/σ(I)>	12.1
Completeness [%]	98.1	93.6	99.2
Redundancy	2.1	2.2	2.1
CC(1/2)		0.997	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2 M ammonium sulfate, 0.1 M Tris pH 8.0