5YCK

Crystal structure of a MATE family protein derived from Camelina sativa at 2.3 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.650, 69.240, 116.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.623 - 2.300
R-factor	0.2256
Rwork	0.223
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjj
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.623	45.623	2.440
High resolution limit [Å]	2.300	6.890	2.300
Rmerge	0.403	0.117	2.451
Rmeas	0.418	0.122	2.545
Total number of observations	274945
Number of reflections	21982	901	3514
<I/σ(I)>	6.48	20.79	1.42
Completeness [%]	97.2	94.4	97.5
Redundancy	12.508	12.433	12.725
CC(1/2)	0.987	0.996	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.1	298	33% PEG300, 200mM (NH4)2SO4, 100mM Tris, 100mM RbCl