5YCH
Ancestral myoglobin aMbWb of Basilosaurus relative (monophyly)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.370, 30.430, 63.330 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 22.399 - 1.354 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ycj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.284 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.400 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.013 | 0.052 |
| Number of reflections | 26897 | 3828 |
| <I/σ(I)> | 41.2 | 18.6 |
| Completeness [%] | 97.3 | 95.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 291 | 76% saturated ammonium sulfate solution containing 2% (w/v) aMbWb by micro-seeding the crashed SWMb crystal |
