5YCH
Ancestral myoglobin aMbWb of Basilosaurus relative (monophyly)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.370, 30.430, 63.330 |
Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
Resolution | 22.399 - 1.354 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ycj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.284 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.400 | 1.430 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.013 | 0.052 |
Number of reflections | 26897 | 3828 |
<I/σ(I)> | 41.2 | 18.6 |
Completeness [%] | 97.3 | 95.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 291 | 76% saturated ammonium sulfate solution containing 2% (w/v) aMbWb by micro-seeding the crashed SWMb crystal |