5YCG
Ancestral myoglobin aMbWp of Pakicetus relative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-19 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.390, 29.740, 58.360 |
| Unit cell angles | 90.00, 112.65, 90.00 |
Refinement procedure
| Resolution | 23.631 - 2.400 |
| R-factor | 0.1862 |
| Rwork | 0.184 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ycj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.554 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.631 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | 0.088 |
| Number of reflections | 7124 | 1034 |
| <I/σ(I)> | 22.9 | 21.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 3.5 M ammonium sulfate solution for a 0.5 mL reservoir, and a mixture of 2 uL of the reservoir solution and 2 uL of protein solution in a 50 mM Tris-HCl (pH 8.0) buffer containing 2% (w/v) aMbWp |
