5YCE
Sperm whale myoglobin swMb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.180, 30.660, 63.570 |
| Unit cell angles | 90.00, 105.49, 90.00 |
Refinement procedure
| Resolution | 9.949 - 0.770 |
| R-factor | 0.1448 |
| Rwork | 0.144 |
| R-free | 0.16150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rgk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.529 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 0.810 |
| High resolution limit [Å] | 0.770 | 0.770 |
| Rmerge | 0.138 | |
| Number of reflections | 148979 | |
| <I/σ(I)> | 20.5 | 5.6 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 4.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 291 | 76% saturated ammonium sulfate |
