5YC2

Crystal structure of inner membrane protein Bqt4 in complex with telomeric protein Rap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	32.154, 51.293, 79.669
Unit cell angles	94.92, 100.56, 105.00

Refinement procedure

Resolution	39.056 - 2.704
R-factor	0.2044
Rwork	0.202
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybx
RMSD bond length	0.002
RMSD bond angle	0.481
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.056	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.324
Rmeas		0.391
Rpim		0.216
Number of reflections	12761
<I/σ(I)>	1.7	2.428
Completeness [%]	98.0	94.8
Redundancy	1	2.9
CC(1/2)		0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v Polyethyleneglycol 4000.