5YBY

Structure of human Gliomedin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	46.880, 81.573, 66.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.257 - 1.429
R-factor	0.151
Rwork	0.150
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xav
RMSD bond length	0.009
RMSD bond angle	1.096
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.429	1.429
Rmerge	0.062	0.519
Rmeas	0.068	0.567
Rpim	0.027	0.226
Number of reflections	47683	4698
<I/σ(I)>	40.2	3.67
Completeness [%]	99.9	99.9
Redundancy	6	6.1
CC(1/2)		0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	ammonium sulfate, Tris-HCl