5YBX

Crystal structure of the N-terminal domain of Bqt4 in S.pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97845
Spacegroup name	I 21 3
Unit cell lengths	94.658, 94.658, 94.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.467 - 2.501
R-factor	0.228
Rwork	0.224
R-free	0.26690
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.765
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.467	7.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge		0.085	0.699
Rmeas		0.088	0.717
Rpim		0.022	0.158
Number of reflections	9479	279	247
<I/σ(I)>	1.9
Completeness [%]	99.9	100	100
Redundancy	1	10	10
CC(1/2)		0.997	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	30% PEG4000, 0.2 M Ammonium acetate, 0.1 M Sodium citrate, pH5.6