5YBU
Structure of the KANK1 ankyrin domain in complex with KIF21A peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.648, 51.624, 137.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.335 - 1.890 |
R-factor | 0.2249 |
Rwork | 0.222 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hbd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.227 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2722) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.335 |
High resolution limit [Å] | 1.890 |
Number of reflections | 22149 |
<I/σ(I)> | 29.3 |
Completeness [%] | 99.5 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05M magnesium formate, 21% PEG 3350 |