5YBT

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA-V150L/A232S/M241V in complex with berkeleyone A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-02-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 62
Unit cell lengths	171.569, 171.569, 45.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.528 - 2.350
R-factor	0.2189
Rwork	0.217
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybm
RMSD bond length	0.007
RMSD bond angle	0.931
Data scaling software	Aimless
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	32016
<I/σ(I)>	13.8
Completeness [%]	98.6
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, lithium citrate