5YBS
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA-V150L/A232S in complex with berkeleyone A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-02-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 62 |
Unit cell lengths | 171.452, 171.452, 45.794 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.494 - 2.300 |
R-factor | 0.2155 |
Rwork | 0.213 |
R-free | 0.26860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ybm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.923 |
Data scaling software | Aimless |
Phasing software | PHENIX ((1.10.1_2155)) |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.500 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 34292 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 98.9 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, lithium citrate |