5YBP

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA-V150L/A232S in complex with preaustinoid A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 62
Unit cell lengths	171.803, 171.803, 45.913
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.595 - 2.312
R-factor	0.2212
Rwork	0.218
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybm
RMSD bond length	0.007
RMSD bond angle	0.945
Data scaling software	Aimless
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	33586
<I/σ(I)>	15.2
Completeness [%]	97.9
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, lithium citrate