5YBN

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA in complex with (alpha)ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-11-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 62
Unit cell lengths	172.350, 172.350, 45.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.087 - 2.104
R-factor	0.1881
Rwork	0.186
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybm
RMSD bond length	0.006
RMSD bond angle	0.810
Data scaling software	Aimless
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	44951
<I/σ(I)>	17.1
Completeness [%]	99.6
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, lithium citrate