5YBL

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AusE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-06-14
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	C 2 2 2
Unit cell lengths	220.342, 223.923, 53.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.414 - 2.108
R-factor	0.2166
Rwork	0.214
R-free	0.26570
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.977
Data scaling software	Aimless
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.414	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	75992
<I/σ(I)>	7.4
Completeness [%]	98.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000, sodium citrate