5YBJ

Structure of apo KANK1 ankyrin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	42.903, 47.602, 137.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.990 - 2.341
R-factor	0.214
Rwork	0.212
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.229
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2722)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.990
High resolution limit [Å]	2.340
Number of reflections	12363
<I/σ(I)>	28.1
Completeness [%]	99.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1M HEPES, pH 7.5, 0.2M Lithium sulfate monohydrate, 25% PEG 3350