5YBD

X-ray structure of ETS domain of Ergp55 in complex with E74DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	39.279, 233.871, 80.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.978 - 2.769
R-factor	0.2081
Rwork	0.204
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.265
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.820
High resolution limit [Å]	2.769	7.510	2.770
Rmerge	0.078	0.058	0.456
Number of reflections	9898
<I/σ(I)>	11.7
Completeness [%]	99.9	98.4	100
Redundancy	6.4	5.4	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	20%(w/v) PEG4000, 200mM MgCl2 as precipitant solution