5YBB

Structural basis underlying complex assembly andconformational transition of the type I R-M system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2016-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41
Unit cell lengths	121.600, 121.600, 280.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.002 - 3.200
R-factor	0.2376
Rwork	0.236
R-free	0.27170
RMSD bond length	0.008
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	66781
<I/σ(I)>	27.8
Completeness [%]	95.3
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M Tris-HCl, 54% MPD, 0.2 M NH4H2PO4, pH 8.8