5YB9
Crystal structure of a dimeric cyclophilin A from T.vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 38.505, 38.505, 365.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.254 - 2.276 |
| R-factor | 0.2189 |
| Rwork | 0.212 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.254 | 2.380 |
| High resolution limit [Å] | 2.270 | 2.300 |
| Rmerge | 0.415 | |
| Number of reflections | 8106 | |
| <I/σ(I)> | 6.18 | 2.107 |
| Completeness [%] | 90.0 | 95.1 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 100 mM HEPES pH 7.0, 30% (v/v) Jeffamine M-600 |
