5YB4

Crystal structure of HP23LN36KR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	51.280, 51.280, 142.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.150 - 2.500
R-factor	0.1916
Rwork	0.188
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.902
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.150
High resolution limit [Å]	2.500
Number of reflections	7140
<I/σ(I)>	15.8
Completeness [%]	100.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	(A12) 10%(w/v) PEG 8000, 1M HEPES, PH=7.5, 8%(v/v) Ethylene glycol 13 (B1) 8%(w/v) PEG 4000, 1M Sodium acetate PH=4.6