5YB0
Crystal Structure of Wild Type Phosphoserine aminotransferase (PSAT) from E. histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2011-04-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.446, 134.073, 139.064 |
| Unit cell angles | 62.85, 88.58, 74.92 |
Refinement procedure
| Resolution | 50.150 - 2.940 |
| R-factor | 0.267 |
| Rwork | 0.265 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bt4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.553 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.150 | 3.050 |
| High resolution limit [Å] | 2.940 | 3.000 |
| Rmerge | 0.083 | 0.881 |
| Number of reflections | 76771 | |
| <I/σ(I)> | 14.911 | |
| Completeness [%] | 94.2 | 85.8 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 25-30% PEG 3350 (w/v), 100 mM Tris pH 6.5, 200mM sodium formate, 5 % glycerol (v/v) |
