5YAY

Crystal structure of KANK1/KIF21A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	38.043, 52.080, 136.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.484 - 1.550
R-factor	0.1841
Rwork	0.183
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hbd
RMSD bond length	0.012
RMSD bond angle	1.353
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.057	0.039	0.835
Rmeas	0.064	0.044	0.929
Rpim	0.028	0.021	0.397
Number of reflections	39577	1798	2005
<I/σ(I)>	15.1
Completeness [%]	98.2	81	100
Redundancy	5.4	4.5	5.5
CC(1/2)		0.996	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M ammonium formate and 20% w/v polyethylene glycol 3350