5YAH
Crystal 2 for AtLURE1.2-AtPRK6LRR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.706, 43.347, 77.821 |
| Unit cell angles | 90.00, 124.70, 90.00 |
Refinement procedure
| Resolution | 31.992 - 2.104 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26580 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.225 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.992 | 2.240 |
| High resolution limit [Å] | 2.104 | 2.104 |
| Number of reflections | 15868 | |
| <I/σ(I)> | 30.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MgCl2, Bis-Tris pH 5.5, PEG 3350 |
