5YAE
Ferulic acid esterase from Streptomyces cinnamoneus at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 2.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.270, 71.290, 87.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.320 - 2.400 |
| R-factor | 0.18248 |
| Rwork | 0.180 |
| R-free | 0.22026 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.320 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.400 |
| Rmerge | 0.070 | 0.190 |
| Number of reflections | 14597 | |
| <I/σ(I)> | 42.9 | 10.6 |
| Completeness [%] | 93.9 | 87.9 |
| Redundancy | 30.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, sodium cacodylate trihydrate (pH 6.5), PEG 8000, glycerol |
