5YAC
Crystal structure of WT Trm5b from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-13 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.444, 88.567, 95.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.150 - 3.300 |
| R-factor | 0.24386 |
| Rwork | 0.242 |
| R-free | 0.28847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hjm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.150 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.204 | 0.980 |
| Number of reflections | 5639 | 812 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 12% (w/v) PEG 3350, 100 mM HEPES (pH 7.5), 100 mM Ca(OAc)2 and 100 mM KCl |
