5YAA

Crystal structure of Marf1 NYN domain from Mus musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97916
Spacegroup name	H 3
Unit cell lengths	163.470, 163.470, 56.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.941 - 1.750
R-factor	0.2346
Rwork	0.232
R-free	0.28270
RMSD bond length	0.009
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	81.740
High resolution limit [Å]	1.750
Number of reflections	56376
<I/σ(I)>	2.152
Completeness [%]	97.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Li2SO4, 0.1M Tris-HCl pH 8.5, 22% PEG3350