5YA6
Crystal structure of archaeal flagellin FlaB1 from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.090, 73.660, 107.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.500 |
| R-factor | 0.17408 |
| Rwork | 0.173 |
| R-free | 0.20000 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.815 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.325 | |
| Number of reflections | 46269 | |
| <I/σ(I)> | 12.2 | 3.3 |
| Completeness [%] | 96.8 | 92.9 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | 32.5% PEG4000 |
