5Y9W
Crystal 1 for AtLURE1.2-AtPRK6LRR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.687, 48.511, 146.310 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 35.383 - 1.847 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mn8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.946 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.383 |
| High resolution limit [Å] | 1.847 |
| Number of reflections | 56086 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 98.2 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M MgCl2, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
